The cytoprotective co-chaperone, AtBAG4, supports increased nodulation and seed protein content in chickpea without yield penalty

Drought and extreme temperatures significantly limit chickpea productivity worldwide. The regulation of plant programmed cell death pathways is emerging as a key component of plant stress responses to maintain homeostasis at the cellular-level and a potential target for crop improvement against environmental stresses. Arabidopsis thaliana Bcl-2 associated athanogene 4 (AtBAG4) is a cytoprotective co-chaperone that is linked to plant responses to environmental stress. Here, we investigate whether exogenous expression of AtBAG4 impacts nodulation and nitrogen fixation. Transgenic chickpea lines expressing AtBAG4 are more drought tolerant and produce higher yields under drought stress. Furthermore, AtBAG4 expression supports higher nodulation, photosynthetic levels, nitrogen fixation and seed nitrogen content under well-watered conditions when the plants were inoculated with Mesorhizobium ciceri. Together, our findings illustrate the potential use of cytoprotective chaperones to improve crop performance at least in the greenhouse in future uncertain climates with little to no risk to yield under well-watered and water-deficient conditions.

Accordingly, to improve the salinity tolerance of soybean (Glycine max L.), Robert et al. expressed the nematode cell death inhibitor CED-9 16 .The transgenic plants displayed superior salinity tolerance, but reduced nodulation upon rhizobia inoculation 16 .Robert et al. postulated that CED-9 inhibits nodule organogenesis by interacting with vesicular trafficking and plant autophagy proteins 16 .Due to the poor nodulation phenotype, it was suggested that the expression of animal PCD suppressors such as CED-9 may be an unsuitable strategy to increase the stress tolerance of nitrogen fixing legume crops 16 .
The B cell lymphoma 2 (Bcl-2)-associated athanogene (BAG) family of co-chaperones is a multifunctional group of evolutionarily conserved proteins that participate in diverse cellular functions including stress responses, proliferation, migration, and cell death 14,17 .In Arabidopsis thaliana seven genes encode BAG proteins, among them, Arabidopsis thaliana BAG4 (AtBAG4) demonstrates the most potential for crop improvement 8,14,17,18 .Transgenic expression of AtBAG4 in tobacco, rice, banana, and cotton improves abiotic stress tolerance including drought and suppresses plant PCD 14,[18][19][20] .Recent research by Locascio et al. also demonstrated a role for AtBAG4 in ion flux control and stomatal movement via regulation of KAT1 potassium channels 21 .Research in mammalian systems demonstrates a role for BAG proteins within autophagy [22][23][24] .Before assessing the suitability of transgenically expressing AtBAG4 as a strategy for improving chickpea cultivation, it is important to investigate the implications of AtBAG4 on chickpea nodulation in well-watered conditions.
Here, we show that transgenic chickpeas expressing AtBAG4 are more drought tolerant and produce higher yields under stress than non-transgenic controls.Mesorhizobium ciceri inoculated chickpea expressing AtBAG4 form equivalent or more nodules compared to non-transgenic control plants.The additional nodules translated to increased shoot nitrogen content for several lines.Moreover, we observed no detrimental effect of AtBAG4 expression on seed yield under irrigated conditions.Taken together, our findings suggest that AtBAG4 is a suitable candidate gene to improve drought stress tolerance in chickpea without yield penalty under irrigated conditions.Furthermore, unlike CED-9, the expression of AtBAG4 does not detrimentally affect nodule formation or nitrogen fixation under irrigated conditions.

Transgenic plants overexpressing AtBAG4 are water-deficit stress tolerant
In the field, plants are subjected to multiple stresses, simultaneously.Previous studies show that transgenic expression of cell death suppressors confers broad-spectrum stress tolerance in plants [9][10][11][12][13]15 . Expession of AtBAG4 provides UV irradiation, oxidants, drought, salinity and cold tolerance in tobacco, rice, and banana [18][19][20] .To determine whether AtBAG4 expression supported increased seed number per plant in chickpea, transgenic chickpea expressing AtBAG4 and non-transgenic control plants were grown until the flowering stage and subjected to water-deficit stress.As shown in Fig. 1, the total number of seeds per plant and the 100 seed weight of the transgenic lines were significantly higher than the non-transgenic controls under water-deficit stress (Fig. 1).When well-watered, the transgenic lines performed as well as the non-transgenic controls and the number of seeds per plant and 100 seed weight was not significantly different between the transgenic and non-transgenic lines (Fig. 1).Thus, no apparent metabolic cost was observed when expressing AtBAG4 in chickpea.

AtBAG4-expressing transgenic plants nodulate at higher or similar levels compared to the non-transgenic controls upon M. ciceri inoculation
Previous studies showed that expression of the cell death suppressor CED-9 improved Glycine max L. salinity tolerance but inhibited Bradyrhizobium japonicum nodulation 16 .To determine the effects of transgenic AtBAG4 on nodule formation, we inoculated four transgenic chickpea lines (AtBAG4-1, AtBAG4-2, AtBAG4-3, AtBAG4-4) expressing AtBAG4 with M. ciceri and compared the number of nodules per plant, on a dry weight basis, against non-transgenic controls.Transgenic AtBAG4-3 (306 nodules per plant) and AtBAG4-4 (217 nodules per plant) lines showed significantly more nodules than the non-transgenic controls (109 nodules per plant).Transgenic AtBAG4-1 and AtBAG4-2 produced an equivalent number of nodules to the non-transgenic controls (Supplementary Fig. 1).As expected, the non-inoculated controls did not nodulate.
We then determined whether nodulation correlated with AtBAG4 expression by quantitative real-time reverse transcription PCR (qRT-PCR) analysis.The expression of AtBAG4 varied in the different chickpea lines (Supplementary Fig. 2).AtBAG4 expression in the AtBAG4-3 transgenic line correlated with the water-deficit stress tolerance and higher nodule number per plant.However, this was not true for the other transgenic lines (Figs. 1, 2 and Supplementary Figs. 1, 2).The difference between phenotype and AtBAG4 expression levels may be due to the T-DNA insertion site or that transcript levels do not always translate at the protein level because of post-transcriptional regulation.
Nodule morphology was similar in the transgenic lines and the controls (Fig. 2B).For all plants, nodule formation occurred primarily on the upper part of the root system, consistent with a properly functioning autoregulation of nodulation (AON) system 25 , and the nodules were pink in colour, indicating the presence of leghaemoglobin.To determine whether AtBAG4 expression affected rhizobial colonisation, we cross-sectioned the nodules for observation under a light microscope (Fig. 2B).Infected cells were densely packed within the infection zone and we observed no clear differences between non-transgenic and transgenic chickpea nodules (Fig. 2B).We conclude that the transgenic chickpeas expressing AtBAG4 develop morphologically equivalent nodules to the non-transgenic controls.www.nature.com/scientificreports/M. ciceri-inoculated transgenic chickpeas expressing AtBAG4 fix more nitrogen and contain high seed protein contents than the non-transgenic controls Since AtBAG4-3 and AtBAG4-4 displayed improved nodulation, these two lines were used for further investigation to determine whether the increased nodulation equated to increased fixation and nitrogen content.Transgenic and non-transgenic plants were inoculated with M. ciceri and the shoot of each plant was harvested, dried, weighed and ground into a powder.A subsample of the powder was then used to analyse the delta 15 N signature of the shoot using IRMS.Nitrogenase enzyme discriminates between N isotopes favouring 14 N over 15 N. Therefore, N fixing systems are enriched for 14 N.All inoculated plants had negative delta 15 N in the shoot biomass to demonstrate active nitrogen fixation and likely reflect the effects of this positive discrimination of nitrogenase for 14 N and/or and discrimination effects during the translocation of fixed nitrogen to the shoot 26,27 (Supplementary Fig. 3).Next, we evaluated the nitrogen yield in these plants.AtBAG4-3 and AtBAG4-4 contained statistically higher nitrogen yield (4.03% and 3.7% respectively) compared to the non-transgenic controls (3.3%) upon M. ciceri inoculation (Fig. 3C).We observed a significant difference between the morphology of the inoculated and non-inoculated chickpea plants.Non-inoculated plants displayed symptoms of nitrogen deficiency, including leaf yellowing and stunted growth whereas inoculated plants were green, attaining typical plant height (Fig. 3A,B).The transgenic plants expressing AtBAG4 performed better than the non-transgenic controls under nitrogen stress, consistent with a possible role of BAG4 in autophagic protein turnover and nitrogen efficiency.Furthermore, the seed N content of the transgenic chickpea lines is significantly higher than non-transgenic controls (Fig. 3D).

Transgenic chickpea showed higher photosynthetic rates upon inoculation with M. ciceri
Nitrogen fixation is one of the most energy expensive processes performed in plants 28,29 .Previously, we showed that AtBAG4 expression supports higher photosynthetic levels in rice 20 .To investigate whether the increased nodulation in transgenic chickpea expressing AtBAG4 was facilitated by access to increased energy resources, we measured and compared the photosynthetic capacity of the transgenic lines and non-transgenic controls.Like our rice data, the rhizobia inoculated chickpea lines expressing AtBAG4 showed significantly higher net photosynthesis rates than the non-transgenic controls under irrigated conditions (Fig. 4).The non-inoculated transgenic chickpea plants are significantly stressed and therefore did not photosynthesise as much as their inoculated counterparts; we observed no significant differences between the non-inoculated transgenic lines and non-transgenic controls (Fig. 4).

Nodule suppressive CLE peptides of chickpea are induced following M. ciceri infection
To ensure a balance between nitrogen acquisition and energy expenditure, the AON pathway tightly regulates nodule formation and nitrogen fixation (Ferguson et al. 25 ).In a negative feedback loop, nodule suppressing CLE peptides (RIC1 and RIC2) are expressed in the root following rhizobial inoculation.CLE peptides have been identified in several model plants 5,25,[30][31][32][33] , but there is little known about the nodulation-suppressing CLE peptides in chickpea.To evaluate whether RIC genes play a role in determining the nodulation of AtBAG4 expressing chickpea, we analysed the expression levels of CaRIC1 and CaRIC2 after 2 h and 5 days of rhizobia inoculation.Expression levels of the housekeeping gene EF1A, were used for normalisation.Here, we observed that the CaRIC1 and CaRIC2 are induced by the compatible rhizobia strain M. ciceri, indicating that they are triggered during nodule organogenesis, with an expression pattern consistent with that reported for their respective orthologues in soybean and bean 32,34 .In both transgenic and non-transgenic plants, CaRIC1 and CaRIC2 gene expression levels were low 2 h post-inoculation and rose significantly by five days post-inoculation.CaRIC2 expression was higher than CaRIC1 after five days of rhizobia inoculation.Coinciding with high photosynthetic rates and increased nodule numbers, CLE gene expression in AtBAG4-3 and AtBAG4-4 plants was significantly lower than observed in non-transgenic control plants (Fig. 5).

Discussion
Chickpea is one of the most widely grown legume crops worldwide, however, production is significantly hampered by drought and extreme temperatures (heat and frost at flowering) 35 .The suppression of plant PCD pathways can improve crop fitness in stressful environments, however, due to potentially deleterious effects on rhizobium symbiosis and nodulation, and plant development, doubts remain to whether PCD suppression is a suitable strategy to improve the agronomic performance of legumes.The Arabidopsis BAG family of co-chaperones is one of the few cell death regulators of plant cell death that are conserved in mammals and a potential target for biotechnological improvement of crops.Previous research has shown that transgenic expression of AtBAG4 improves the agronomic performance of rice, tobacco and banana [18][19][20] .Although our knowledge of the molecular mechanisms driving AtBAG4-mediated tolerance remain incomplete, we recently revealed that AtBAG4 interacts with the selective autophagy substrate NBR1, and autophagy is required for AtBAG4-mediated stress tolerance (Under review).Here we used a combination of reverse genetics, expression analysis and stress assays to demonstrate that chickpeas expressing AtBAG4 are drought tolerant, as evidenced by increased yields under stressed conditions.We also show that under well-watered conditions, transgenic AtBAG4-expressing chickpea formed nodules when inoculated with M. ciceri and fixed atmospheric nitrogen at similar or higher levels than the non-transgenic controls.
Contrary to expressing other cell death regulators, such as CED-9 and BI-1 in legumes, the expression of AtBAG4 improved stress tolerance without affecting nodulation and nitrogen fixation detrimentally under well-watered conditions.The different outcome between AtBAG4 and other cell death suppressors may be due to the molecular roles that each of the proteins play and the fact that CED-9 and BI-1 increase autophagy.Although, autophagy is required for nodulation due to its role in host defence and cellular homeostasis 36,37 , modulating the pathways too much can have detrimental effects.For example, during nodule organogenesis, the host plant expresses nodulins to establish plant-rhizobia symbiosis 36 .Silencing of nodulins increases the expression of autophagy genes, including Beclin-1/ATG6, ATG8, VPS15 and PI3K, leading to more defective nodules 36 .Conversely, autophagy is required for the symbiotic relationship between P. vulgaris and symbiotic microorganisms 37 .Downregulation of Beclin1 in P. vulgaris results in less nodulation 37 .
In plants, the complete conservation of apoptosis pathways remains doubtful, however, plants expressing CED-9 display reduced cell death and suppressed apoptotic-like hallmarks to suggest that mammalian apoptosis pathways are at least partially conserved in plants.Due to the lack of plant apoptotic-machinery, previous studies suggest that in plants, CED-9 suppresses cell death, by regulating autophagy pathways, via interaction with Beclin-1 38 .Similarly, Bax inhibitor 1 (BI-1) also interacts with ATG6/Beclin-1 in plants and positively regulates autophagy 39 .Expression of Bax-inhibitor I (BI-I) in common bean root increases the number of rhizobium infections and nodulation, however, the nodules die prematurely 40 .Given the vital role of autophagy within symbiotic relationships 36 , we hypothesise that the interaction of CED-9 and BI-1 with Beclin-1 and subsequent regulation of autophagy pathways may have a detrimental effect on nodule formation in legumes.In contrast, AtBAG4 is a co-chaperone potentially involved in chaperone-mediated selective autophagy.Although, autophagy www.nature.com/scientificreports/pathways are involved in AtBAG4-mediated stress tolerance, AtBAG4 does not play a major regulatory role in plant autophagy pathways.This subtle difference between the roles of AtBAG4 and CED-9 and BI-1 may help explain why the expression of AtBAG4 did not detrimentally affect on chickpea nodulation.Potassium homeostasis is crucial to efficient regulation of stomatal movement.The proper regulation of potassium ion flux enables plants to respond rapidly to environmental conditions and prevent excessive water loss, specifically under drought and salinity conditions 41 .Previous research has shown a role for AtBAG4 in potassium homeostasis regulation via interaction with the potassium ion channel KAT1 21 .KAT1 help regulate potassium fluxes in the guard cell and to manipulate stomatal opening 42 .By directly interacting with KAT1, AtBAG4 positively regulates the KAT1 potassium channel to modulate stomatal movement 21 .The regulation of stomatal aperture has an essential role in drought stress tolerance and photosynthesis.Our results showed that AtBAG4 expressing plants maintain higher photosynthetic levels under irrigated conditions, this is likely due to AtBAG4 expression positively regulating stomatal aperture, thus facilitating efficient gas exchange to optimise the compromise between photosynthesis and water management.Although not tested in this study, the better photosynthetic control may also allow the AtBAG4 expressing plants to better manage stress tolerance and nodulation.Biological nitrogen fixation and nodule formation are resource-intensive processes 28,43 .Irrigated AtBAG4 expressing transgenic chickpea plants produce more energy via higher net photosynthesis to support nodulation possibly via regulation of AON pathway.During subsequent drought stress, these resources may better support drought stress tolerance.The increased energy capacity in transgenic plants helps to improve the water use efficiency under drought stress therefore, it prevents excessive water loss and provides drought stress tolerance.
Legume nodulation is regulated by systemic mechanisms and local host defence pathways in response to rhizobia interaction.In contrast, the premature cell death observed following AtBI-I expression, is thought to occur due to activation of plant defence systems that induce plant cell death 40,44 .The cell death response in BI-1 expressing plants may also be attributed to BI-1's interaction with Beclin-1 which is an established player in the hypersensitive response of tobacco to infection with Tobacco mosaic virus (TMV) 45 .
The elicitation of the plant defence limits the number of infection events in the roots and therefore contributes to determining the number of nodules formed in the legume root.During the rhizobia infection, roots cells may identify some rhizobia as pathogens.A universal response of plants to pathogen attack is an oxidative burst and cell death at the site of pathogen penetration 46 .By improving stress tolerance and ROS detoxification, AtBAG4 expression may help attenuate cell death associated with plant defence to allow more rhizobia to survive and subsequently increase nodulation.Specifically, in combination with autophagy pathways, AtBAG4 may help suppress the accumulation of unfolded proteins, which can result in cell death if left unchecked.By reducing the levels of cell death associated with rhizobia infection, AtBAG4 subsequently supports increased nodulation (Fig. 6).Increased nodule numbers lead to higher shoot nitrogen content in rhizobia inoculated transgenic chickpea (Fig. 6).

Conclusion and future perspectives
We showed that expression of AtBAG4, a cytoprotective co-chaperone, is a promising strategy to improve legume stress tolerance while not affecting nodulation, nitrogen fixation or yield under well-watered conditions.Further studies are however needed to understand the effect of AtBAG4 expression on chickpea nodulation, net photosynthesis, and nitrogen fixation under water-deficit stress.Future studies should also investigate the relationship between, nodule formation, nodule senescence and CLE peptide signalling in chickpea, potentially leading to advances in improving chickpea nodulation in agriculture.

Plant growth conditions
Independent transgenic chickpea lines of the commercially available HatTrick cultivar constitutively expressing a single copy of the AtBAG4 transgene were generated previously by Tropical Pulses for Queensland (TPFQ) project at the Queensland University of Technology and the transgenic seeds for this study were kindly provided by TPFQ team 47 .Transgenic chickpea seeds were surface sterilised with 1% sodium hypochlorite for three minutes with vigorous shaking.Following sterilisation, the seeds were thoroughly rinsed four to five times with sterile milliQ water and placed on plates containing sterile wet filter paper for germination at 23 °C in the dark.Three days post-germination, the seedlings were placed in 4L pots containing grade 3 sterile vermiculite (for nodulation assay) or soil (for yield assay).Plants were watered (200 mL) every second day and supplemented with nitrogen-free B & D solution once a week 48 .Unless otherwise stated, all plants were grown at 23 °C for 16 h light and 8 h dark cycle with 40-60% relative humidity.Non-transgenic chickpea was used as controls.

Bacterial growth conditions
For nodulation, Mesorhizobium ciceri strain CC1192 was grown for 48 h at 28 °C in Yeast Mannitol Broth (YMB), harvested and diluted with nitrogen-free B&D solution to a final concentration of OD 600 ~ 0.1 49 .Each pot was treated with 150 mL of diluted M.ciceri.As controls, a complete set of non-inoculated plants from each genotype, including non-transgenic controls, was supplied with nitrogen-free B&D solution.

Drought stress assay
All drought experiments were conducted in the PC2 (Physical containment level 2) glasshouse at QCDF Redland, Queensland, Australia.Non-transgenic and three transgenic chickpea lines were planted in pots (165 mm diameter × 240 mm height) containing 4 kg of soil (1:1 potting mix and sand) with a randomised statistical block design.A total of three replicates with three seedlings per replicate (total nine plants) of each line were planted www.nature.com/scientificreports/ in pots for the drought treatments and irrigated controls.All plants were irrigated with 200 mL water three times per a week to maintain the soil relative water content above 50% of field capacity until early flowering stage (47 days post-sowing).For drought treatment, the plants were not watered after the 47th day until senescence.As irrigated controls, plants were supplied 200 ml of water three times per week until pod maturity before withholding irrigation completely for pod drying.The number of seeds per plant and 100 seed weight of each line were counted under drought stress and irrigated conditions.

Nodulation assay
Three-day old seedlings were transferred to pots containing sterile vermiculite and treated with 150 mL of M. ciceri inoculum.Five plants were grown in each pot and five biological replicates were assessed for each genotype.
Triplicate experiments were performed.Plants were grown for 21 days before nodule harvesting and recording of the number of nodules per plant.Plants were watered every second day with equal amounts of water added to each pot.The plants were dried separately at 60 °C for two days before measuring the dry weights.showed that BAG4 directly binds with KAT1 and aids in K + fluxes in the guard cells and results in regulating the stomatal opening 21 .Higher nodulation leads to higher nitrogen content in transgenic shoots and accumulated in seeds as indicated by higher seed protein content in transgenic lines.Increased nodulation under full irrigated conditions was supported by high energy capacity in transgenic chickpea plants.Another possible reason for increased nodulation in transgenic plants is the modulation of defence response upon rhizobia infection.Higher autophagy levels in AtBAG4 overexpressing plants suppress cell death and defence response in roots.Therefore, more rhizobia infected cells will survive to form more nodules.Increased nodulation leads to increased nitrogen content in the shoot under nitrogen depleted conditions.Therefore, AtBAG4 expression potentially increases the nitrogen use efficiency in transgenic chickpea plants.Furthermore, our results showed that there is no yield penalty in rhizobia inoculated AtBAG4 expressing transgenic chickpea lines under well-watered conditions.Overall, this study suggests that transgenic expression of AtBAG4 in chickpea a good strategy to improve chickpea cultivation in future. Vol.:(0123456789)

Light microscopy
Nodules were harvested after 21 days, fixed in 0.05% paraformaldehyde, and stored at 4 °C in 70% ethanol until sectioning.The nodules were processed for five hours and twenty minutes using a Leica ASP300S tissue processor before embedding into paraffin.Samples were bisected longitudinally through the nodules to obtain cross sections of both the nodule and the root.These were placed face down in the cassette (Thermofisher Shandon Histocentre 3 Embedding Station).Thin nodule sections were made using Leica RM2245 microtome followed by heat fixing for 40 min at 60 °C.Following fixation, the slides were stained with 0.1% Toluidine Blue O and scanned using a 3D Histech Panoramic Scan II on the 40X objective.For the detailed procedure see Supplementary Table 1.

Isotope analysis
M. ciceri-inoculated and non-inoculated chickpea shoots (2 months old) were harvested, dried for two days at 60 °C and ground with a planetary cylinder mill.The samples were analysed for N content and delta 15 N signatures using an Isotope ratio mass spectrometer IRMS (20-22 Sercon Limited, UK).Five biological replicates were analysed from each genotype.

Net photosynthesis measurements
Inoculated and non-inoculated chickpea plants grown in the glasshouse were used for the photosynthesis measurements.Net photosynthesis was measured using a LiCOR Infra-Red Gas Analyser LI-6400 XT (John Morris Scientific, Chatwood, NSW, Australia) according to the manufacturer's instructions.The third newest fully grown compound leaf of each plant was used for the measurement and all the measurements were taken before flowering.Ten biological replicates were used for each genotype.

RNA extraction and cDNA synthesis
For CLAVATA/ESR-related (CLE) peptide analysis, three-day old seedlings were planted in pots containing sterile vermiculite and grown for five days.Plants were watered every second day with sterile distilled water.M. ciceri inoculum was added to each pot eight days post-germination 34 .The entire root system was harvested 0 and 5 days post-inoculation, snap frozen in liquid nitrogen and stored at − 80 °C until RNA extraction using a plant RNeasy kit (Qiagen) according to the manufacturer's instructions.To remove contaminating genomic DNA from the sample, approximately 1 μg of total RNA was treated with 1U of RQ1 DNase (Promega) at 37 °C for 30 min.The DNase reaction was terminated by the addition of 1μL of RQ1 DNase stop solution and incubation at 65 °C for 10 min.cDNA was synthesized using a GoScript Reverse transcription system (Promega) according to the manufacturer's instructions.For AtBAG4 gene expression analysis, leaf samples were collected from two month old glasshouse grown plants and snap frozen.RNA was isolated using plant RNeasy kit according to the manufacturer's instructions similar procedure was followed as above.

Quantitative real time PCR
Quantitative real time PCR was performed according to the methods described in Hayashi et al. 50.Primers for AtBAG4, CaRIC1 and CaRIC2 genes were designed using Primer3 bioinformatic software (MIT) software 51 .A list of primers used in this study is provided in Table S2.CaEF1A was used as a housekeeping gene to normalise the AtBAG4, CaRIC1 and CaRIC2 transcript abundance.

Statistical analysis
All data were subjected to one-way ANOVA (MINITAB 18 Statistical Software).Differences among means for treatments were evaluated by Tukey pairwise comparison test at 0.05 probability levels.
All the methods were carried out in accordance with Queensland University of Technology guidelines and regulations.

Figure 1 .
Figure 1.Transgenic expression of AtBAG4 improved water-deficit stress tolerance in the greenhouse.(A) Number of seeds per plant under well-watered and water-deficient conditions.(B) Hundred seed weight of each genotype under well-watered and water-deficit stress conditions.Non-transgenic (HatTrick) and three transgenic chickpea lines (AtBAG4-1, AtBAG4-2, AtBAG4-3) were grown in the glasshouse to the flowering stage and then subjected to water-deficit stress.For the well-watered controls, the plants were watered every second day.Error bars indicate the standard error.Different letters and * represent statistically significant differences determined with ANOVA followed by Tukey's test between transgenic and non-transgenic plants (n = 3, p ≤ 0.05).

Figure 2 .
Figure 2. Transgenic lines expressing AtBAG4 showed higher nodulation compared to the non-transgenic control under well-watered conditions.(A) Average nodule number per plant in transgenic and non-transgenic chickpeas.Error bars indicate the standard error.Different letters represent statistically significant differences determined with ANOVA followed by Tukey's test between transgenic and non-transgenic plants (n = 5, p ≤ 0.05).(B) Nodule morphology of transgenic and non-transgenic chickpeas.

Figure 3 .
Figure 3. Transgenic AtBAG4 expressing chickpea display enhanced shoot growth and accumulate more nitrogen.(A) and (B) Plant morphology of inoculated and non-inoculated chickpea plants respectively.(C) Nitrogen content in shoot tissue.(D) Seed protein content.Error bars indicate the standard error.Different letters and * represent statistically significant differences determined with ANOVA followed by Tukey's test between transgenic and non-transgenic plants (n = 5, p ≤ 0.05).

Figure 5 .
Figure 5. Expression of the nodulation CLE peptide genes, CaRIC1 and CaRIC2 in transgenic and nontransgenic chickpea.Values show the relative transcript abundance of CaRIC1 and CaRIC2 at 2 h and 5 days following the application of M. ciceri.Expression levels were determined using quantitative real-time reverse transcription PCR.Error bars indicate the standard error.Different letters and * represent statistically significant difference determined with ANOVA followed by Tukey's test between transgenic and non-transgenic plants (n = 3, p ≤ 0.05). https://doi.org/10.1038/s41598-023-45771-3

Figure 6 .
Figure 6.Model of how AtBAG4 confers drought stress tolerance in chickpea without affecting yield and nodulation.Transgenic chickpea expressing AtBAG4 showed higher photosynthesis under well-watered conditions.Locascio et al. showed that BAG4 directly binds with KAT1 and aids in K + fluxes in the guard cells and results in regulating the stomatal opening21 .Higher nodulation leads to higher nitrogen content in transgenic shoots and accumulated in seeds as indicated by higher seed protein content in transgenic lines.Increased nodulation under full irrigated conditions was supported by high energy capacity in transgenic chickpea plants.Another possible reason for increased nodulation in transgenic plants is the modulation of defence response upon rhizobia infection.Higher autophagy levels in AtBAG4 overexpressing plants suppress cell death and defence response in roots.Therefore, more rhizobia infected cells will survive to form more nodules.Increased nodulation leads to increased nitrogen content in the shoot under nitrogen depleted conditions.Therefore, AtBAG4 expression potentially increases the nitrogen use efficiency in transgenic chickpea plants.Furthermore, our results showed that there is no yield penalty in rhizobia inoculated AtBAG4 expressing transgenic chickpea lines under well-watered conditions.Overall, this study suggests that transgenic expression of AtBAG4 in chickpea a good strategy to improve chickpea cultivation in future.